Biodiversity refers to variety and variability (takes into account all taxonomic levels from kingdom to subspecies or race) among the various life forms and ecological complexes in which they meet. However, biodiversity is also defined as the variability of living resources, in their relationships with the environment in which they live. Specific diversity is generally considered to be one of the key factors in ecosystem resilience in response to anthropogenic pressures, including fisheries. In this context, we analyzed the spatio-temporal structure and the diversity of demersal fish species on the continental shelf of Benin. There is now a growing awareness of the role of the marine environment for the population and the imperative to maintain its balance and preserve its wealth. Face to this obligation , the management of the ecosystem of this environment becomes necessary. Fishing has changed the biological and dynamic characteristics of exploited species. For example, changes in density and distribution, a decrease in the average size of individuals, or a decrease in age at first sexual maturity lead to a decrease in biomass. Diversity index is the measure of species diversity in a given community. It is different from species richness in that unlike richness it also shows community composition and takes into account the relative abundance of species that are present in the community. The Regional Fish Stock Assessment Project (PRESH), a component of the WAEMU Concerted Fisheries and Aquaculture Management Plan, aims to improve and strengthen the state of knowledge of the potential of the fisheries resources of the countries concerned. Scientific knowledge of fish stocks will enable decisions to be made to ensure the rational and sustainable exploitation of fisheries. Within the framework of the WAEMU Agricultural Policy (PAU), the three-year program for the development of the fisheries sector in WAEMU was adopted in Dakar in March 2003, the objective of which is to establish a process of coordination and harmonization of the management of shared fishery resources, with a view to sustainable management of these resources and contributing to food security and the reduction of poverty in the WAEMU area. This program includes, among other things, the definition of a cooperative management plan for fisheries and aquaculture within the UEMOA, underpinned by a good knowledge of the state of the fishery resources in UEMOA countries. The first phase of this program was conducted in 2012 with the assessment of pelagic fish stocks. During this survey in all countries, it was decided to make an assessment of the demersal stocks. For example, the Guinean research vessel N/O "General Lansana Conté" was selected to conduct demersal resource assessment surveys in Côte d'Ivoire, Ghana, Togo and Benin. Global objective of this survey were to assess demersal resources and follow up biomass and indices. Specific objective of this study is just to analyze biodiversity composition. Materials and Methods The survey is conducted onboard the CNSHB's fishing N/O research vessel, "General Lansana Conté". This research vessel manufactured in Japan is a trawler of the rear-fishing type with the Overall length: 29.93 m, Long: 27.15 m, Breadth: 7.30 m, Gross tonnage: 198 tonnage gauge (tx) Net tonnage: 59 tx. To estimate trawl opening size, distance measurements were made between the fishing pulleys and the spacing between the boars. These parameters are taken after trawl spinning, at each fishing operation. This calculation method makes possible to obtain average values which differ from one stratum to another. At the end of each trawl tow, fishes was sorted, the weight of the catches and the measurement of the fish caught are carried out. The weighing of the catches was carried out using two scales: one digital biggest (type "Marel M1100e" with a maximum capacity of 60 kg) and the second digital small (type SPRING-DIAL HOIST CALE, of 50 kg). The 50 kg mid-range scale was used for individual weighing of small and medium-sized specimens, while the 60 kg electronic scale was used for group weighing and large individuals.The measurement of the individuals was carried out using types of wooden graduated ichthyometers (up to 50 cm in length and 100 cm). Each of these strata, according to depths and area, was also subdivided into squares of 3 nautical miles from each other (Fig. 1). Fig. 1. Location of trawling stations in the three bathymetric strata During the survey a Stratified Random Sampling (SRS) plan was adopted with diurnal trawling with tow duration of 30 minutes (from the complete stop of the winch). The allocation of the stratum was carried out by weighting according to the surface area of the continental shelf (2.731 km²). The squares thus obtained were drawn at random and those retained contain a trawling station. Twenty-one stations were programmed and realized, in 3 days, in the depths ranging from 10 to 100 m. In the coastal stratum (10-25 m), nine stations were trawled (42.95%), the intermediate zone (25-50 m) included 5 stations (29.31%) and the deep zone (50-100 m) - 7 stations (27.74%). Fish biodiversity in most rapid assessments that have been carried out so far has been assessed as a key part of surveys which target a number of important taxa in an area. Given the characteristics of the Beninese seabed, a map was made available to the captain in order to avoid obstacles and risk areas as much as possible. For the purposes of this cruise, trawling on board was conducted at an average speed of 3.5 knots. Data collected During the survey, four (04) types of data were collected: - meteorological data (wind speed and direction); - environmental data (surface and bottom temperatures and surface salinity); - catches by weight and number for all species caught; - measurements (lengths) for some targeted species. Data entry was done under Access where some estimates were made and other estimates in Excel. Sampling of environmental data. The CTD probe was only used at three stations because of a failure reported by the Chief Scientist. The sea surface temperature was collected by a thermal sensor (surface thermometer) while the bottom temperature was obtained through the net attached to the trawl. Catches sampling. After each trawling tow, the entire catch was sorted and distributed by species for a numerical and weight inventory. In the case of big catches, only a fraction is kept for sorting. This fraction, can be equal to 1/2, 1/3 or 1/4 of the total catch, is sorted and weighed, then reduced to the total catch (before capture). This method has the advantage of providing a representative sub-sample insofar as the species present are numerous. When in the catch, we observe a big specimen of fish, we separate them from the catch before sampling. After the weighing and counting of the catches, the individuals in the sample belonging to groups of fish species (or other taxa) retained for size frequency monitoring were measured. Size frequency collection was performed by total length (cm) for fish and coat length (cm) for cuttlefish. The species that were measured during this campaign are nineteen (19) including 18 species of fish and cuttlefish. These selected species are usually followed in Benin because of their commercial importance. In this study, we are interested in data on living resources. Estimation procedure The formulas used to calculate the indicators provided by this demersal trawl survey were as follows. The average catch (index of abundance) by tow () of a species or group of species in a given area or stratum is estimated using the following formula: (1) where Xi - trawl catch; i and n - numbers of tows. Average of catches or abundance indices observed and abundances presented are relative values (Catch per Unit of Effort) calculated from the ratio of the sum of total catches to the number of trawl stations in the stratum for each species caught. They are expressed in kilograms per 30 minutes of trawling. The area swept (a) by a haul of trawl was calculated using the following formula: where d - distance traveled by the trawl during a haul, km; oh - horizontal opening of the trawl, km; v - tow speed, km/h; t - trawl time, 0,5 h. The biomass (B) of a species or group of species in a given area or stratum is estimated using the following formula: where - the average catch; A - area of the area or stratum; a - area swept by the trawl; k - proportion of fish caught in the swept area (usually 0.5 ≤ k ≤ 1, Sparre, P. & Venema, SC, 1996 ). For this study, the value of k is equal to 1, given that the trawl collects everything on its course. The mean length () of a length frequency sample is estimated using the following formula: where n - total number of individuals; k - number of length classes; fj - number of individuals of length class j; xj - center of the class of length j. The density (biomass per unit area) of a species or group of species d = B/A, where B is biomass, kg or t, and A - swept area, km². It is the biomass per unit area. Unit: kg/km² or t/km². Biodiversity measuring There are many ways to measure Biodiversity on a site, but we will retain only the most used ones that answer the following questions: - how many species are found on this site? (specific wealth); - how important is each species on this site? (Abundance-dominance); - do the different species have similar numbers? (digital regularity); - how to globally quantify species Biodiversity on this site (diversity indices). Taxonomic richness Intuitively, our first perception of the environment tells us that the more distinct taxa (species, genera, families, ...), the greater the diversity. Taxonomic richness is simply the number of distinct taxa on an inventory list and answers the first question. Abundance - Dominance. Abundance is the number of individuals surveyed for a given taxon. Dominance is a note indicating the proportion of area (or volume) covered by a taxon (especially for species). Indeed, a species can "dominate" a site by its coverage while its workforce on this site is low. Diversity. To simultaneously quantify the taxonomic richness and distribution of taxa in a community, diversity indices are frequently used, the three main ones being Shannon-Weaver, Simpson's and Equitability . We use here Simpson’s index expressed par formula below: Shannon Weather index. Shannon Index is a commonly used diversity index that takes into account both abundance and evenness of species present in the community. It is explained by the following formula: where H' - biodiversity index; S is the number of taxa present in the survey and pi is the proportion of taxon i in the survey; ln is the natural log; Σ is the sum of the calculations; pi = ni/N where ni is the number of individuals of the species and N is the total number of individuals, all species combined. Shannon’s equitability (EH) measures the evenness of a community and can be easily calculated by diving the value of H with Hmax, which equals to lnS (S - number of species encountered). Its value ranges between 0 and 1, with being complete evenness: Results and discussion The distribution of total catches by zoological group shows that bone fish are by far the most abundant during this survey with 86.49% of total catches, followed by cartilaginous fish, 6.34% of catches (Table 1). Table 1 Distribution of catches by zoological group Zoological Groups Catch, kg Percentage, % Osteichthyans (bone fish) 1 257,81 86,49 Chondrichthyes (cartilaginous fish) 92,2 6,34 Cephalopods 56,76 3,90 Crustaceans 47,46 3,26 Total 1 454,23 100 Cephalopods and crustaceans represent only 3.90 and 3.26% of total catches, respectively. The relative importance of these bony fish, plus cartilaginous fish (Smooth Pollock Shark, Rays and Angelfish), can be explained by the fact that the fishing gear used is a standard fish trawl. In catches, the most abundant family is the Sparidae with 18% of the total weight followed by Polynemidae, 13% and Carangidae, 10%. The proportion of all other families is below 10%. The first three most dominant species in the catches are the Angolan tooth (Dentex angolensis) with 9% of the total weight followed by the gray grouper or thiof (Epinephelus aeneus) and the ordinary disc (Drepane africana) with 8%. The proportions of all other species are below 8%. This proportion decreases gradually to 1%, if we consider the top twenty species of taxonomy (Table 2). Table 2 First twenty taxa Taxa Catch, kg Weight, % Dentex angolensis 123.80 9 Epinephelus aeneus 119.45 8 Drepane africana 118.90 8 Polydactylus quadrifilis 96.50 7 Galeoides decadactylus 94.30 6 Pseudotolithus senegalensis 67.00 5 Chloroscombrus chrysurus 64.20 4 Sphyraena guachancho 62.40 4 Sparus caeruleostictus 56.65 4 Sepia officinalis hierredda 52.61 4 Decapterus punctatus 51.15 4 Squatina oculata 43.50 3 Dentex congoensis 41.55 3 Brachydeuterus auritus 37.88 3 Raja miraletus 30.60 2 Pseudotolithus typus 30.00 2 Dasyatis centroura 26.50 2 Balistes capriscus 23.40 2 Pagellus bellottii 23.10 2 Priacanthus arenatus 21.50 1 It should be noted that of these first twenty species, only three do not have much commercial interest; they are the triggerfish (Balistes capriscus), the sun-like beauclairs (Priacanthus arenatus) and the Ocellated Sea Angel (Squatina oculata), which used to be discards. Today, there are practically no more rejects because of the decline in production; all species are consumed by the population. This tendency is shown on Table 2. We observe three big classes on the dendrogram (Fig. 2). Fig. 2. First twenty taxa The chart below is the dendrogram. It represents how the algorithm works to group the observations, then the sub groups of observations. The algorithm has successfully grouped all the observations. The dotted line represents the automatic truncation, leading to three groups. Two groups are approximately the same size, and the third one is a big size. The first two groups (displayed in blue and red color) are more homogeneous than the third one. This is confirmed when looking at the Within-class variable. It is a lot higher for the third group than for the first one (Fig. 2). The first result to look at is the levels bar chart. The shape reveals a great deal about the structure of the data. When the increase in dissimilarity level is strong, we have reached a level where we are grouping groups that are already homogenous. Automatic truncation uses this criterion to decide when to stop aggregating observations (or groups of observations) (Fig. 3). Fig. 3. Levels bar Chart Abundance indexes The tables below show the Catch Per Unit Effort (CPUE), the CPUE per stratum, and the abundance indexes by bathymetric strata. The total abundance index obtained (Table 3) for all species combined is 69.25 kg/tow at 21 trawl stations (the duration of a tow is 30 minutes). Table 3 Abundance indexes EEZ Total weight, kg Number of individuals Number of tows CPUE, kg/tow CPUE, individuals/tow Values 1 454.23 415 21 69.25 20 This abundance index varies between the 3 strata as follows (Table 4): - the 10-25 m stratum with 80.25 kg/tow; - the 25-50 m stratum for 39.46 kg/tow; - the 50-100 m stratum for 82.54 kg/tow. Table 4 Average catches or abundance indexes per bathymetric strata Stratum 10-25 m 25-50 m 50-100 m Catchs, kg 722.24 236.75 495.22 Number of stations 09 06 06 Number of individuals 174 108 133 CPUE, kg/tow 80.25 39.46 82.54 CPUE, individuals/tow 19 18 22 Occurrence The table below shows the most common species (Table 5). Table 5 Species occurrences Taxons Number of stations where the species were found Occurrence, % Sepia officinalis hierredda 15 71.43 Chylomycterus reticulatus 14 66.67 Dentex canariensis 14 66.67 Sparus caeruleostictus 13 61.90 Decapterus punctatus 12 57.14 Raja miraletus 11 52.38 Sphyraena guachancho 10 47.62 Epinephelus aeneus 9 42.86 Pagellus bellottii 9 42.86 Aluterus blankerti 8 38.10 Brachydeuterus auritus 8 38.10 Dasyatis margarita 8 38.10 Fistularia petimba 8 38.10 Priacanthus arenatus 8 38.10 Torpedo torpedo 8 38.10 The occurrence of species i (ni%) is the ratio of the number of stations where the species was caught to the total number of stations. The total number of stations is 21. Sepia officinalis hierredda has the highest rate of occurrence (71.43%) followed by Chylomycterus reticulatus with (66.67%) and Dentex canariensis with (66.67%). Among the commercially important fish species Dentex canariensis has the highest rate (66.67%) followed by Sparus caeruleostictus (61.90%) (Table 5). Species richness Data collected and analyzed during the survey identified 100 taxa in the three areas surveyed. The demersal species represent the majority of the species caught with 80.78% of the total weight whereas the pelagic species represent 15.12%. In stratum 10-25 m there are 45 species, 39 in stratum 25-50 m and 35 in stratum 50-100 m. The total catch in the three areas explored is 1 454.23 kg for 21 952 individuals, all species combined. This catch is distributed as shown in Table 6 below. Table 6 Total catch of different groups species Groupe species Nb Taxa Catch, kg Weight, % Bottom 77 1174.76 80.78 Pelagic 13 219.9 15.12 Cephalopods 4 55.61 3.82 Crustaceans 6 3.96 0.27 Total 100 1 454.23 100 The species grouping the two categories (demersal and pelagic) represent weight of 1 394.66 kg (or 95.9% of the total catch). Crabs are represented by 2.65 kg for 102 individuals. Shrimp are represented by a single species of 2.65 kg for 102 individuals. Sea cucumbers are represented by sea cucumber (echinoderm) of 1 kg for 1 individual found on a station. The squils total a weight of 0.15 kg for 3 individuals and lobsters, 1 kg for 6 individuals. In general, during this survey in Benin waters, it has been listed the following specific indicators: A hundred taxa including 76 demersal species (69 bony and 7 cartilaginous fish) and 13 pelagic species consisting entirely of bony fish. Species richness according to the trawling stations varies between 28 and 7 species. It is maximum in stratum 25-50 m and station number 10 represents 7.76% of the total, followed by station 19 with 26 taxa in stratum 50-100 m. The lowest taxon station is station 4 on stratum 10-25m with 4 taxa representing 1.94% of all targeted taxa (Table 7). Table 7 Taxa richness Station number Species number Percentage, % Weight, kg Depth, m Stratum 10 28 7.76 55.33 44.20 25-50 19 26 7.20 47.32 66.90 50-100 20 24 6.65 160.90 67.00 50-100 21 24 6.65 116.85 69.70 50-100 15 23 6.37 75.71 39.00 25-50 9 22 6.09 47.20 15.00 10-25 18 21 5.82 41.05 61.00 50-100 5 20 5.54 59.55 18.00 10-25 14 20 5.54 44.55 57.80 50-100 16 18 4.99 50.01 50.00 50-100 17 18 4.99 84.55 89.80 50-100 6 17 4.71 18.02 18.00 10-25 7 15 4.16 128.95 16.30 10-25 12 14 3.88 18.96 35.70 25-50 11 13 3.60 19.05 26.30 25-50 8 12 3.32 3.70 23.00 10-25 13 12 3.32 17.68 26.30 25-50 1 11 3.05 343.80 13.70 10-25 2 9 2.49 64.00 15.30 10-25 3 7 1.94 34.52 17.00 10-25 4 7 1.94 22.50 15.8 10-25 Shannon Weaver and Simpson index Before discussing the stability properties of the system (here, in terms of biodiversity), it is necessary to characterize the ecological situation . The level of description of this study is that of the standard coastal ichthyological. Equitability assessment is useful for detecting changes in the structure of a community and has sometimes proven effective in detecting anthropogenic changes. The measure of equitability corresponding to the Shannon-Weaver index is carried out according to formula (1). Generally and independently of the taxonomic group, the Shannon-Weaver index is between less than 1 and 4.5; rarely more. A value close to Hʹ = 0.5 is already very weak. H' is minimal (= 0), if all individuals in the stand belong to a single species, it is also minimal if, in a stand each species is represented by a single individual, except one species that is represented by all other individuals of the stand. The index is maximal when all individuals are evenly distributed across all species . The Shannon index is often accompanied by the Piélou equitability index (1966) , also called equidistribution index , which represents the ratio of H' to the theoretical maximum index in the stand. (Hmax). This index can vary from 0 to 1, it is maximal when the species have identical abundances in the stand and it is minimal when a single species dominates the whole population. Insensitive to specific richness, it is very useful for comparing potential dominance between stations or between sampling dates. For each stratum, the Shannon index is less than 4.5; this means that all individuals are evenly distributed over all species . The Simpson's index close to 0, varying between 0 and 0.049, shows a great diversity especially in the first stratum of 10 to 25 m this trend reinforces the results of the 2012 study [9, 10]. This result reinforces the hypothesis that the coastal zone is a spawning area where several species come to feed  (Table 8). Table 8 Indexes per stratum Stratum* H' E VarH' Simpson D ST1 3.881 596 438 0.956 0 0.000 865 0.000 000 00 ST2 3.689 621 989 0.948 0 0.001 611 0.049 972 36 ST3 3.597 764 412 0.939 7 0.001 706 0.042 035 03 ALL STATION 4.318 616 922 0.937 8 0.000 891 0.015 202 84 * ST1 - Stratum 10-25 m ; ST2 - Stratum 25-50 m; ST3 - Stratum 50-100 m. Shannon's specific diversity index does not evolve in the same direction as equitability. This is explained by the intense degradation of these environments where one or two species dominate by their recovery the other species present. This index therefore seems to be of little use in arid zones and is not very relevant for explaining faunal diversity in extreme cases. Difference between stratums is not too significant according to the tab of variance analysis. (Table 9, 10). Fisher test used here explain that probability Pr associated to F is less than 0,568; this mean that we take a risk to make a mistake less than 56,8%. Table 9 Correlation matrix Stratum Stratum-ALL STATION Stratum-ST1 Stratum-ST2 Stratum-ST3 H' Stratum ALL STATION 1 -0.333 -0.333 -0.333 0.929 Stratum-ST1 -0.333 1 -0.333 -0.333 0.020 Stratum-ST2 -0.333 -0.333 1 -0.333 -0.379 Stratum-ST3 -0.333 -0.333 -0.333 1 -0.570 H' 0.929 0.020 -0.379 -0.570 1 Table 10 Analysis of variance (H') Source DF Sum of squares Mean squares F Pr > F Model 3 47.331 15.777 1.219 0.568 Error 1 12.944 12.944 - - Corrected Total 4 60.274 - - - Frequency of occurrence was determined for each species over the total 21 stations. Dominant species for each station was determined using catch rates (number of catch per haul) for each stratum, Shannon-Weather index of diversity (S-W index, Hʹ), species richness (S) [12, 13]. This index will have a value of 0 to indicate the maximum diversity and a value of 1 to indicate the minimum diversity. In order to obtain "more intuitive" values, the Simpson diversity index represented by 1-D may be preferred, the diversity maximum being represented by the value 1, and the diversity minimum by the value 0 . Conclusion The study of biodiversity through synthetic indices, as we have done, consists in studying an ecosystem through emerging indicators of the state of diversity of its biocenosis. The impact of fishing on abundance (in biomass) and on species richness is quite important but it seems to be offset by an increase in numbers for most species. Use of these indexes is pertinent to evaluate richness diversity mainly Shannon index. Such a study, although it does not seem sufficient to evaluate the "ecological state" of the system, may nonetheless be interesting in the context of a precautionary approach . It makes it possible to approach the systemic aspect of the management of exploited marine resources because, as Frontier (1999)  points out, "taxonomic diversity does not faithfully reflect functional diversity, but still remains, from a holistic point of view, an indicator of the complexity of the system. This study is not complete because doing just for a few days data. It’s better to make the study during a long period, may be one year. This study allow just to have an overview of the stand and the consequences of the significant increase of fishing pressure on the stand and not only to focus on a few populations of species of commercial interest.